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Native Electrospray and Electron-Capture Dissociation in FTICR Mass Spectrometry Provide Top-Down Sequencing of a Protein Component in an Intact Protein Assembly

The intact yeast alcohol dehydrogenase (ADH) tetramer of 147 kDa was introduced into a FTICR mass spectrometer by native electrospray. Electron capture dissociation of the entire 23+ to 27+ charge state distribution produced the expected charge-reduced ions and, more unexpectedly, 39 c-type peptide...

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Bibliografiske detaljer
Main Authors: Zhang, Hao, Cui, Weidong, Wen, Jianzhong, Blankenship, Robert E., Gross, Michael L.
Format: Artigo
Sprog:Inglês
Udgivet: 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2991543/
https://ncbi.nlm.nih.gov/pubmed/20843701
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jasms.2010.08.006
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