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Role of glutamic acid-181 in DNA-sequence recognition by the catabolite gene activator protein (CAP) of Escherichia coli: altered DNA-sequence-recognition properties of [Val181]CAP and [Leu181]CAP.

It has been proposed that Glu-181 of the catabolite gene activator protein (CAP) makes direct contact with certain base pairs of the specific DNA site. We have purified wild-type CAP and two substituted CAP variants, [Val181]CAP and [Leu181]CAP, and have assessed the DNA-sequence-recognition propert...

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Dettagli Bibliografici
Pubblicato in:Proc Natl Acad Sci U S A
Autori principali: Ebright, R H, Kolb, A, Buc, H, Kunkel, T A, Krakow, J S, Beckwith, J
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 1987
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC299012/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2888111/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.17.6083
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