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How Cations Can Assist DNase I in DNA Binding and Hydrolysis

DNase I requires Ca(2+) and Mg(2+) for hydrolyzing double-stranded DNA. However, the number and the location of DNase I ion-binding sites remain unclear, as well as the role of these counter-ions. Using molecular dynamics simulations, we show that bovine pancreatic (bp) DNase I contains four ion-bin...

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Detalhes bibliográficos
Main Authors: Guéroult, Marc, Picot, Daniel, Abi-Ghanem, Joséphine, Hartmann, Brigitte, Baaden, Marc
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2987838/
https://ncbi.nlm.nih.gov/pubmed/21124947
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pcbi.1001000
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