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Fidelity of DNA polymerases in DNA amplification.
Denaturing gradient gel electrophoresis (DGGE) was used to separate and isolate the products of DNA amplification by polymerase chain reaction (PCR). The strategy permitted direct enumeration and identification of point mutations created by T4, modified T7, Klenow fragment of polymerase I, and Therm...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1989
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC298472/ https://ncbi.nlm.nih.gov/pubmed/2594764 |
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