Purification of Recombinant Acyl-Coenzyme A:Cholesterol Acyltransferase 1 (ACAT1) from H293 Cells and Binding Studies Between the Enzyme and Substrates Using Difference Intrinsic Fluorescence Spectroscopy

Acyl-coenzyme A:cholesterol acyltransferase 1 (ACAT1) is a membrane bound enzyme utilizing long-chain fatty acyl-coenzyme A and cholesterol to form cholesteryl esters and coenzyme A. Previously, we had expressed tagged human ACAT1 (hACAT1) in CHO cells and purified it to homogeneity; however, only a...

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Detalhes bibliográficos
Main Authors: Chang, Catherine CY, Miyazaki, Akira, Dong, Ruhong, Kheirollah, Alireza, Yu, Chunjiang, Geng, Yong, Higgs, Henry N, Chang, Ta-Yuan
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2982893/
https://ncbi.nlm.nih.gov/pubmed/20964445
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi1013936
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