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Isolation of the alpha subunits of GTP-binding regulatory proteins by affinity chromatography with immobilized beta gamma subunits.

Immobilized beta gamma subunits of GTP-binding regulatory proteins (G proteins) were used to isolate alpha subunits from solubilized membranes of bovine tissues and to separate specific alpha subunits based on their differential affinities for beta gamma subunits. The beta gamma subunits were cross-...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Pang, I H, Sternweis, P C
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1989
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC298161/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2510152/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.20.7814
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