Nucleofection disrupts tight junction fence function to alter membrane polarity of renal epithelial cells

Here, we compared the effects of nucleofection and lipid-based approaches to introduce siRNA duplexes on the subsequent development of membrane polarity in kidney cells. Nucleofection of Madin-Darby canine kidney (MDCK) cells, even with control siRNA duplexes, disrupted the initial surface polarity...

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Bibliografiske detaljer
Main Authors: Mo, Di, Potter, Beth A., Bertrand, Carol A., Hildebrand, Jeffrey D., Bruns, Jennifer R., Weisz, Ora A.
Format: Artigo
Sprog:Inglês
Udgivet: American Physiological Society 2010
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Articles
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2980404/
https://ncbi.nlm.nih.gov/pubmed/20702601
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1152/ajprenal.00152.2010
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