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Nucleofection disrupts tight junction fence function to alter membrane polarity of renal epithelial cells
Here, we compared the effects of nucleofection and lipid-based approaches to introduce siRNA duplexes on the subsequent development of membrane polarity in kidney cells. Nucleofection of Madin-Darby canine kidney (MDCK) cells, even with control siRNA duplexes, disrupted the initial surface polarity...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Physiological Society
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2980404/ https://ncbi.nlm.nih.gov/pubmed/20702601 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1152/ajprenal.00152.2010 |
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