Nalaganje...
Design and creation of a Ca2+ binding site in human lysozyme to enhance structural stability.
A Ca2+ binding site like an EF-hand motif was designed and created in human lysozyme by replacing both Gln-86 and Ala-92 with aspartic acids by site-directed mutagenesis. The mutant human lysozyme (D86/92-lysozyme) was expressed and secreted by yeast. One Ca2+ was found to bind one molecule of the p...
Shranjeno v:
| Main Authors: | , , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
1989
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC297958/ https://ncbi.nlm.nih.gov/pubmed/2674939 |
| Oznake: |
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