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Secretion in yeast of human lysozymes with different specific activities created by replacing valine-110 with proline by site-directed mutagenesis.

Computer graphics indicate that a steric hindrance exists between valine-110 side chain of human lysozyme (EC 3.2.1.17) and an acetyl group of a modified substrate that contains N6,O-diacetylmuramic acid. To alter the substrate specificity of human lysozyme to be effective on the modified substrate,...

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Detalhes bibliográficos
Main Authors: Kikuchi, M, Yamamoto, Y, Taniyama, Y, Ishimaru, K, Yoshikawa, W, Kaisho, Y, Ikehara, M
Formato: Artigo
Idioma:Inglês
Publicado em: 1988
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC282762/
https://ncbi.nlm.nih.gov/pubmed/3059346
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