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Expression and purification of ELP-intein-tagged target proteins in high cell density E. coli fermentation
BACKGROUND: Elastin-like polypeptides (ELPs) are useful tools that can be used to non-chromatographically purify proteins. When paired with self-cleaving inteins, they can be used as economical self-cleaving purification tags. However, ELPs and ELP-tagged target proteins have been traditionally expr...
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| Auteurs principaux: | , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
BioMed Central
2010
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2978133/ https://ncbi.nlm.nih.gov/pubmed/20959011 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-9-77 |
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