Automated DNA Extraction, Quantification, Dilution, and PCR Preparation for Genotyping by High-Resolution Melting

Genotyping by high-resolution amplicon melting uses only two PCR primers per locus and a generic, saturating DNA dye that detects heteroduplexes as well as homoduplexes. Heterozygous genotypes have a characteristic melting curve shape and a broader width than homozygous genotypes, which are usually...

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Detaylı Bibliyografya
Asıl Yazarlar: Seipp, Michael T., Herrmann, Mark, Wittwer, Carl T.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Association of Biomolecular Resource Facilities 2010
Konular:
Communications
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC2977966/
https://ncbi.nlm.nih.gov/pubmed/21119928
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