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Digital PCR for direct quantification of viruses without DNA extraction
DNA extraction before amplification is considered an essential step for quantification of viral DNA using real-time PCR (qPCR). However, this can directly affect the final measurements due to variable DNA yields and removal of inhibitors, which leads to increased inter-laboratory variability of qPCR...
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| Publié dans: | Anal Bioanal Chem |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Springer Berlin Heidelberg
2015
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4706837/ https://ncbi.nlm.nih.gov/pubmed/26483186 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-015-9109-0 |
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