Characterization and functional reconstitution of a soluble form of the hydrophobic membrane protein lac permease from Escherichia coli.
Lac permease, a polytopic membrane protein from Escherichia coli, has been purified in soluble form by overexpressing the lacY gene by means of the T7 RNA polymerase system. Soluble permease is dissociated from membranes with urea or other chaotropes and appears after the membrane is saturated with...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1989
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC297780/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2668955/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.16.6087 |
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