The iron-responsive element binding protein: a method for the affinity purification of a regulatory RNA-binding protein.
A method of affinity purification of a regulatory protein that binds specific RNA sequences is described. RNAs containing the regulatory sequences are transcribed in vitro from oligonucleotide templates, biotinylated, and incubated with unfractionated cytosol. Specific RNA-protein complexes are boun...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1989
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC297711/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2474819/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.15.5768 |
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