The iron-responsive element binding protein: a method for the affinity purification of a regulatory RNA-binding protein.

A method of affinity purification of a regulatory protein that binds specific RNA sequences is described. RNAs containing the regulatory sequences are transcribed in vitro from oligonucleotide templates, biotinylated, and incubated with unfractionated cytosol. Specific RNA-protein complexes are boun...

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Bibliografiske detaljer

Udgivet i:	Proc Natl Acad Sci U S A
Principais autores:	Rouault, T A, Hentze, M W, Haile, D J, Harford, J B, Klausner, R D
Format:	Artigo
Sprog:	Inglês
Udgivet:	National Academy of Sciences 1989
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC297711/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2474819/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.15.5768
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