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Intracellular methicillin selection of Listeria monocytogenes mutants unable to replicate in a macrophage cell line.

To dissect the determinants of Listeria monocytogenes that are required for pathogenicity, we designed an intracellular selection protocol based on penicillin selection to isolate mutants defective for intracellular growth. Eight independent mutants obtained by insertion of Tn916 were isolated that...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Camilli, A, Paynton, C R, Portnoy, D A
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1989
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC297655/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2501788/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.14.5522
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