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Intracellular methicillin selection of Listeria monocytogenes mutants unable to replicate in a macrophage cell line.

To dissect the determinants of Listeria monocytogenes that are required for pathogenicity, we designed an intracellular selection protocol based on penicillin selection to isolate mutants defective for intracellular growth. Eight independent mutants obtained by insertion of Tn916 were isolated that...

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Bibliografiske detaljer
Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Camilli, A, Paynton, C R, Portnoy, D A
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1989
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC297655/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2501788/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.14.5522
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