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ATP Changes the Fluorescence Lifetime of Cyan Fluorescent Protein via an Interaction with His148

Recently, we described that ATP induces changes in YFP/CFP fluorescence intensities of Fluorescence Resonance Energy Transfer (FRET) sensors based on CFP-YFP. To get insight into this phenomenon, we employed fluorescence lifetime spectroscopy to analyze the influence of ATP on these fluorescent prot...

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Detalhes bibliográficos
Main Authors: Borst, Jan Willem, Willemse, Marieke, Slijkhuis, Rik, van der Krogt, Gerard, Laptenok, Sergey P., Jalink, Kees, Wieringa, Be, Fransen, Jack A. M.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2974648/
https://ncbi.nlm.nih.gov/pubmed/21079777
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0013862
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