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Optimization of ex vivo hematopoietic stem cell expansion in intermittent dynamic cultures
For the ex vivo expansion of CD34(+) cells, culture conditions were optimized using cytokine cocktails and media change methods. In addition, static, orbital-shake, and stirred cultures were compared. After cultivation, total cell expansion, immunophenotypes, clonogenic ability, and metabolite conce...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Springer Netherlands
2010
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2974206/ https://ncbi.nlm.nih.gov/pubmed/20652621 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10529-010-0355-0 |
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