Strategy for complete NMR assignment of disordered proteins with highly repetitive sequences based on resolution-enhanced 5D experiments

A strategy for complete backbone and side-chain resonance assignment of disordered proteins with highly repetitive sequence is presented. The protocol is based on three resolution-enhanced NMR experiments: 5D HN(CA)CONH provides sequential connectivity, 5D HabCabCONH is utilized to identify amino ac...

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Autors principals: Motáčková, Veronika, Nováček, Jiří, Zawadzka-Kazimierczuk, Anna, Kazimierczuk, Krzysztof, Žídek, Lukáš, Šanderová, Hana, Krásný, Libor, Koźmiński, Wiktor, Sklenář, Vladimír
Format: Artigo
Idioma:Inglês
Publicat: Springer Netherlands 2010
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2966349/
https://ncbi.nlm.nih.gov/pubmed/20890634
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-010-9447-3
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