High-quality gene assembly directly from unpurified mixtures of microarray-synthesized oligonucleotides

To meet the growing demand for synthetic genes more robust, scalable and inexpensive gene assembly technologies must be developed. Here, we present a protocol for high-quality gene assembly directly from low-cost marginal-quality microarray-synthesized oligonucleotides. Significantly, we eliminated...

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Detalhes bibliográficos
Main Authors: Borovkov, Alex Y., Loskutov, Andrey V., Robida, Mark D., Day, Kristen M., Cano, Jose A., Le Olson, Tien, Patel, Hetal, Brown, Kevin, Hunter, Preston D., Sykes, Kathryn F.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2010
Assuntos:
Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2965257/
https://ncbi.nlm.nih.gov/pubmed/20693531
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq677
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