Restoration of catalytic functions in Cre recombinase mutants by electrostatic compensation between active site and DNA substrate

Two conserved catalytic arginines, Arg-173 and Arg-292, of the tyrosine site-specific recombinase Cre are essential for the transesterification steps of strand cleavage and joining in native DNA substrates containing scissile phosphate groups. The active site tyrosine (Tyr-324) provides the nucleoph...

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Main Authors: Kachroo, Aashiq H., Ma, Chien-Hui, Rowley, Paul A., Maciaszek, Anna D., Guga, Piotr, Jayaram, Makkuni
Formáid: Artigo
Teanga:Inglês
Foilsithe: Oxford University Press 2010
Ábhair:
Nucleic Acid Enzymes
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2965224/
https://ncbi.nlm.nih.gov/pubmed/20511594
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq466
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