AZIDE-SPECIFIC LABELLING OF BIOMOLECULES BY STAUDINGER-BERTOZZI LIGATION: PHOSPHINE DERIVATIVES OF FLUORESCENT PROBES SUITABLE FOR SINGLE-MOLECULE FLUORESCENCE SPECTROSCOPY

We describe the synthesis of phosphine derivatives of three fluorescent probes that have brightness and photostability suitable for single-molecule fluorescence spectroscopy and microscopy: Alexa488, Cy3B, and Alexa647. In addition, we describe procedures for use of these reagents in azide-specific,...

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Bibliografski detalji
Glavni autori: Chakraborty, Anirban, Wang, Dongye, Ebright, Yon W., Ebright, Richard H.
Format: Artigo
Jezik:Inglês
Izdano: 2010
Teme:
Article
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2965076/
https://ncbi.nlm.nih.gov/pubmed/20580957
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0076-6879(10)72018-8
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