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Overexpression of biotin synthase and biotin ligase is required for efficient generation of sulfur-35 labeled biotin in E. coli
BACKGROUND: Biotin is an essential enzyme cofactor that acts as a CO(2 )carrier in carboxylation and decarboxylation reactions. The E. coli genome encodes a biosynthetic pathway that produces biotin from pimeloyl-CoA in four enzymatic steps. The final step, insertion of sulfur into desthiobiotin to...
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| Autores principales: | , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
BioMed Central
2010
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2964542/ https://ncbi.nlm.nih.gov/pubmed/20937134 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-10-73 |
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