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A Solubilized Elastin Substrate for Continuous Fluorimetric Assay of Kinetics of Elastases
Elastolysis is central to progression of emphysema and aortic aneurysms. Characterization of steady-state enzyme kinetics of elastolysis is fettered by the insolubility of mature elastin and polydispersity of solubilized elastin. We prepared a fluor-tagged, 100 kDa fraction (fEln-100) from commercia...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
2010
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2964420/ https://ncbi.nlm.nih.gov/pubmed/20828532 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2010.09.001 |
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