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Rapid Determination of Enzyme Kinetics from Fluorescence: Overcoming the Inner Filter Effect
Fluorescence change is convenient for monitoring enzyme kinetics. Unfortunately, it looses linearity as the absorbance of the fluorescent substrate increases with concentration. When the sum of absorbance at excitation and emission wavelengths exceeds 0.08, this inner filtering effect (IFE) alters a...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2211277/ https://ncbi.nlm.nih.gov/pubmed/17706587 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2007.07.008 |
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