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Microbiome Profiling by Illumina Sequencing of Combinatorial Sequence-Tagged PCR Products
We developed a low-cost, high-throughput microbiome profiling method that uses combinatorial sequence tags attached to PCR primers that amplify the rRNA V6 region. Amplified PCR products are sequenced using an Illumina paired-end protocol to generate millions of overlapping reads. Combinatorial sequ...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2964327/ https://ncbi.nlm.nih.gov/pubmed/21048977 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0015406 |
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