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A Simple and General Method for Determining the Protein and Nucleic Acid Content of Viruses by UV Absorbance

UV spectra of viruses are complicated by overlapping protein and RNA absorbance and light scattering. We describe and validate methodology for estimating RNA and protein concentration from such spectra. Importantly, we found that encapsidation did not substantially affect RNA absorbance. Combining a...

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Detalhes bibliográficos
Main Authors: Porterfield, J. Zachary, Zlotnick, Adam
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2964155/
https://ncbi.nlm.nih.gov/pubmed/20850162
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.virol.2010.08.015
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