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Rapid and simple method for purification of nucleic acids.

We have developed a simple, rapid, and reliable protocol for the small-scale purification of DNA and RNA from, e.g., human serum and urine. The method is based on the lysing and nuclease-inactivating properties of the chaotropic agent guanidinium thiocyanate together with the nucleic acid-binding pr...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Principais autores: Boom, R, Sol, C J, Salimans, M M, Jansen, C L, Wertheim-van Dillen, P M, van der Noordaa, J
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1990
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC269651/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1691208/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.28.3.495-503.1990
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