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Making temperature-sensitive mutants

The study of temperature sensitive (Ts) mutant phenotypes is fundamental to gene identification and for dissecting essential gene function. In this chapter we describe two “shuffling” methods for producing Ts mutants using a combination of PCR, in vivo recombination, and transformation of diploid st...

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Autors principals: Ben-Aroya, Shay, Pan, Xuewen, Boeke, Jef D., Hieter, Philip
Format: Artigo
Idioma:Inglês
Publicat: 2010
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2957654/
https://ncbi.nlm.nih.gov/pubmed/20946811
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0076-6879(10)70008-2
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