Multicolor Fluorescence Nanoscopy in Fixed and Living Cells by Exciting Conventional Fluorophores with a Single Wavelength

Current far-field fluorescence nanoscopes provide subdiffraction resolution by exploiting a mechanism of fluorescence inhibition. This mechanism is implemented such that features closer than the diffraction limit emit separately when simultaneously exposed to excitation light. A basic mechanism for...

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Detalhes bibliográficos
Main Authors: Testa, Ilaria, Wurm, Christian A., Medda, Rebecca, Rothermel, Ellen, von Middendorf, Claas, Fölling, Jonas, Jakobs, Stefan, Schönle, Andreas, Hell, Stefan W., Eggeling, Christian
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2010
Assuntos:
Spectroscopy, Imaging, and Other Techniques
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2956215/
https://ncbi.nlm.nih.gov/pubmed/20959110
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.08.012
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