Fluorescence Nanoscopy in Whole Cells by Asynchronous Localization of Photoswitching Emitters

We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the p...

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Bibliografiske detaljer
Main Authors: Egner, Alexander, Geisler, Claudia, von Middendorff, Claas, Bock, Hannes, Wenzel, Dirk, Medda, Rebecca, Andresen, Martin, Stiel, Andre C., Jakobs, Stefan, Eggeling, Christian, Schönle, Andreas, Hell, Stefan W.
Format: Artigo
Sprog:Inglês
Udgivet: The Biophysical Society 2007
Fag:
Spectroscopy, Imaging, Other Techniques
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2025649/
https://ncbi.nlm.nih.gov/pubmed/17660318
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.112201
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