Fluorescence Nanoscopy in Whole Cells by Asynchronous Localization of Photoswitching Emitters

We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the p...

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Detalhes bibliográficos
Main Authors: Egner, Alexander, Geisler, Claudia, von Middendorff, Claas, Bock, Hannes, Wenzel, Dirk, Medda, Rebecca, Andresen, Martin, Stiel, Andre C., Jakobs, Stefan, Eggeling, Christian, Schönle, Andreas, Hell, Stefan W.
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2007
Assuntos:
Spectroscopy, Imaging, Other Techniques
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2025649/
https://ncbi.nlm.nih.gov/pubmed/17660318
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.112201
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