FRAP Analysis of Membrane-Associated Proteins: Lateral Diffusion and Membrane-Cytoplasmic Exchange

Obtaining quantitative kinetic parameters from fluorescence recovery after photobleaching (FRAP) experiments generally requires a theoretical analysis of protein mobility and appropriate solutions for FRAP recovery derived for a given geometry. Here we provide a treatment of FRAP recovery for a mole...

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Detalhes bibliográficos
Main Authors: Goehring, Nathan W., Chowdhury, Debanjan, Hyman, Anthony A., Grill, Stephan W.
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2010
Assuntos:
Cellular Biophysics and Electrophysiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2956213/
https://ncbi.nlm.nih.gov/pubmed/20959084
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.08.033
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