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A simplified erythromycin resistance cassette for Treponema denticola mutagenesis
The primary selectable marker for genetic studies of Treponema denticola is a hybrid gene cassette containing both ermF and ermAM (ermB) genes. ErmB functions in Escherichia coli, while ErmF has been assumed to confer resistance in T. denticola. We demonstrate here that ErmB is sufficient for erythr...
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2010
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2953711/ https://ncbi.nlm.nih.gov/pubmed/20691222 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2010.07.020 |
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