PCR-Based Pooling of Dried Blood Spots for Detection of Malaria Parasites: Optimization and Application to a Cohort of Ugandan Children

Sensitive, high-throughput methods to detect malaria parasites in low-transmission settings are needed. PCR-based pooling strategies may offer a solution. We first used laboratory-prepared samples to compare 2 DNA extraction and 4 PCR detection methods across a range of pool sizes and parasite densi...

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Main Authors: Hsiang, Michelle S., Lin, Michael, Dokomajilar, Christian, Kemere, Jordan, Pilcher, Christopher D., Dorsey, Grant, Greenhouse, Bryan
格式: Artigo
語言:Inglês
出版: American Society for Microbiology (ASM) 2010
主題:
Parasitology
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2953134/
https://ncbi.nlm.nih.gov/pubmed/20686079
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00522-10
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