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An Efficient Multistrategy DNA Decontamination Procedure of PCR Reagents for Hypersensitive PCR Applications

BACKGROUND: PCR amplification of minute quantities of degraded DNA for ancient DNA research, forensic analyses, wildlife studies and ultrasensitive diagnostics is often hampered by contamination problems. The extent of these problems is inversely related to DNA concentration and target fragment size...

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Main Authors: Champlot, Sophie, Berthelot, Camille, Pruvost, Mélanie, Bennett, E. Andrew, Grange, Thierry, Geigl, Eva-Maria
Formato: Artigo
Idioma:Inglês
Publicado: Public Library of Science 2010
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2946917/
https://ncbi.nlm.nih.gov/pubmed/20927390
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0013042
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