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An Efficient Multistrategy DNA Decontamination Procedure of PCR Reagents for Hypersensitive PCR Applications

BACKGROUND: PCR amplification of minute quantities of degraded DNA for ancient DNA research, forensic analyses, wildlife studies and ultrasensitive diagnostics is often hampered by contamination problems. The extent of these problems is inversely related to DNA concentration and target fragment size...

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Detalhes bibliográficos
Main Authors: Champlot, Sophie, Berthelot, Camille, Pruvost, Mélanie, Bennett, E. Andrew, Grange, Thierry, Geigl, Eva-Maria
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2946917/
https://ncbi.nlm.nih.gov/pubmed/20927390
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0013042
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