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Epitope tag-induced synthetic lethality between cohesin subunits and Ctf7/Eco1 acetyltransferase
Ctf7/Eco1-dependent acetylation of Smc3 is essential for sister chromatid cohesion. Here, we use epitope tag-induced lethality in cells diminished for Ctf7/Eco1 activity to map cohesin architecture in vivo. Tagging either Smc1 or Mcd1/Scc1, but not Scc3/Irr1, appears to abolish access to Smc3 in ctf...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2946494/ https://ncbi.nlm.nih.gov/pubmed/20728441 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.febslet.2010.08.020 |
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