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Rapid denoising of pyrosequencing amplicon data: exploiting the rank-abundance distribution

We developed a fast method for denoising pyrosequencing for community 16S rRNA analysis. We observe a 2–4 fold reduction in the number of observed OTUs (operational taxonomic units) comparing denoised with non-denoised data. ~50,000 sequences can be denoised on a laptop within an hour, two orders of...

Täydet tiedot

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Bibliografiset tiedot
Päätekijät: Reeder, Jens, Knight, Rob
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2010
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC2945879/
https://ncbi.nlm.nih.gov/pubmed/20805793
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth0910-668b
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