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From Alcohol Dehydrogenase to a “One-way” Carbonyl Reductase by Active-site Redesign: A MECHANISTIC STUDY OF MANNITOL 2-DEHYDROGENASE FROM PSEUDOMONAS FLUORESCENS
Directional preference in catalysis is often used to distinguish alcohol dehydrogenases from carbonyl reductases. However, the mechanistic basis underpinning this discrimination is weak. In mannitol 2-dehydrogenase from Pseudomonas fluorescens, stabilization of (partial) negative charge on the subst...
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| Glavni autori: | , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
American Society for Biochemistry and Molecular Biology
2010
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2945559/ https://ncbi.nlm.nih.gov/pubmed/20639204 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.108993 |
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