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On the role of Brønsted catalysis in Pseudomonas fluorescens mannitol 2-dehydrogenase.
X-ray structure of the Pseudomonas fluorescens mannitol 2-dehydrogenase ternary complex with NAD+ and D-mannitol suggests that Lys-295 provides catalytic base assistance to secondary alcohol group oxidation. We have replaced Lys-295 by site-directed mutagenesis with alanine or methionine and evaluat...
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| Autori principali: | , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2003
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1223657/ https://ncbi.nlm.nih.gov/pubmed/12826012 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20030733 |
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