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On the role of Brønsted catalysis in Pseudomonas fluorescens mannitol 2-dehydrogenase.

X-ray structure of the Pseudomonas fluorescens mannitol 2-dehydrogenase ternary complex with NAD+ and D-mannitol suggests that Lys-295 provides catalytic base assistance to secondary alcohol group oxidation. We have replaced Lys-295 by site-directed mutagenesis with alanine or methionine and evaluat...

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Bibliografiske detaljer
Main Authors: Klimacek, Mario, Kavanagh, Kathryn L, Wilson, David K, Nidetzky, Bernd
Format: Artigo
Sprog:Inglês
Udgivet: 2003
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1223657/
https://ncbi.nlm.nih.gov/pubmed/12826012
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20030733
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