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Kinetic and functional analysis of the small RNA methyltransferase HEN1: The catalytic domain is essential for preferential modification of duplex RNA

The HEN1 RNA methyltransferase from Arabidopsis thaliana catalyzes S-adenosyl-L-methionine (AdoMet)-dependent 2′-O-methylation at the 3′-termini of small double-stranded RNAs and is a crucial factor in the biogenesis of plant small noncoding RNAs, such as miRNAs or siRNAs. We performed functional an...

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Main Authors: Vilkaitis, Giedrius, Plotnikova, Alexandra, Klimašauskas, Saulius
Format: Artigo
Jezik:Inglês
Izdano: Cold Spring Harbor Laboratory Press 2010
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2941102/
https://ncbi.nlm.nih.gov/pubmed/20705645
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2281410
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