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Fluorescence Anisotropy Reveals Order and Disorder of Protein Domains in the Nuclear Pore Complex

We present a new approach for studying individual protein domains within the nuclear pore complex (NPC) using fluorescence polarization microscopy. The NPC is a large macromolecular complex, the size and complexity of which presents experimental challenges. Using fluorescence anisotropy and exploiti...

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Detalles Bibliográficos
Main Authors: Mattheyses, Alexa L., Kampmann, Martin, Atkinson, Claire E., Simon, Sanford M.
Formato: Artigo
Idioma:Inglês
Publicado: The Biophysical Society 2010
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2941012/
https://ncbi.nlm.nih.gov/pubmed/20858414
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.06.075
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