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Converting GLX2-1 into an active glyoxalase II

Arabidopsis thaliana glyoxalase 2-1 (GLX2-1) exhibits extensive sequence similarity with GLX2 enzymes but is catalytically inactive with SLG, the GLX2 substrate. In an effort to identify residues essential for GLX2 activity, amino acid residues were altered at positions 219, 246, 248, 325, and 328 i...

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Bibliografiske detaljer
Main Authors: Limphong, Pattraranee, Adams, Nicole E., Rouhier, Matthew F., McKinney, Ross M., Naylor, Melissa, Bennett, Brian, Makaroff, Christopher A., Crowder, Michael W.
Format: Artigo
Sprog:Inglês
Udgivet: 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2939260/
https://ncbi.nlm.nih.gov/pubmed/20715794
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi1010865
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