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In situ reverse transcription: the magic of strength and anonymity

In this study, we describe an approach that enables a highly specific, effective and fast detection of polyadenylated RNA sequences in situ at the light and electron microscopy levels. The method developed is based on the incorporation of 5-bromo-2′-deoxyuridine into the growing cDNA strand by means...

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Hlavní autoři: Ligasová, Anna, Koberna, Karel
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2010
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2938209/
https://ncbi.nlm.nih.gov/pubmed/20627869
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq619
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