Targeted Quantitation of Site-Specific Cysteine Oxidation in Endogenous Proteins Using a Differential Alkylation and Multiple Reaction Monitoring Mass Spectrometry Approach

Reactive oxygen species (ROS) are both physiological intermediates in cellular signaling and mediators of oxidative stress. The cysteine-specific redox-sensitivity of proteins can shed light on how ROS are regulated and function, but low sensitivity has limited quantification of the redox state of m...

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Bibliografiske detaljer
Main Authors: Held, Jason M., Danielson, Steven R., Behring, Jessica B., Atsriku, Christian, Britton, David J., Puckett, Rachel L., Schilling, Birgit, Campisi, Judith, Benz, Christopher C., Gibson, Bradford W.
Format: Artigo
Sprog:Inglês
Udgivet: The American Society for Biochemistry and Molecular Biology 2010
Fag:
Research
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2938085/
https://ncbi.nlm.nih.gov/pubmed/20233844
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M900643-MCP200
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