Development and Application of Multiprobe Real-Time PCR Method Targeting the hsp65 Gene for Differentiation of Mycobacterium Species from Isolates and Sputum Specimens

We developed a multiprobe real-time PCR assay targeting hsp65 (HMPRT-PCR) to detect and identify mycobacterial isolates and isolates directly from sputum specimens. Primers and probes for HMPRT-PCR were designed on the basis of the hsp65 gene sequence, enabling the recognition of seven pathogenic my...

وصف كامل

محفوظ في:
التفاصيل البيبلوغرافية
المؤلفون الرئيسيون: Kim, Kijeong, Lee, Hyungki, Lee, Mi-Kyung, Lee, Seoung-Ae, Shim, Tae-Sun, Lim, Seong Yong, Koh, Won-Jung, Yim, Jae-Joon, Munkhtsetseg, Bazarragchaa, Kim, Wonyong, Chung, Sang-In, Kook, Yoon-Hoh, Kim, Bum-Joon
التنسيق: Artigo
اللغة:Inglês
منشور في: American Society for Microbiology (ASM) 2010
الموضوعات:
Mycobacteriology and Aerobic Actinomycetes
الوصول للمادة أونلاين:https://ncbi.nlm.nih.gov/pmc/articles/PMC2937694/
https://ncbi.nlm.nih.gov/pubmed/20631094
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00939-10
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