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Use of Amino Acids as Inducers for High-Level Protein Expression in the Single-Protein Production System
By taking advantage of MazF, an ACA codon-specific mRNA interferase, Escherichia coli cells can be converted into a bioreactor producing only a single protein of interest by using an ACA-less mRNA for the protein. In this single-protein production (SPP) system, we engineered MazF by replacing two tr...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2937506/ https://ncbi.nlm.nih.gov/pubmed/20656860 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00815-10 |
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